I possess a strong interest in acquiring knowledge and understanding the underlying principles of various scientific phenomena, including experiments, mechanisms, and scientific instrumentation. This curiosity has facilitated the development of my expertise in a range of instrumental techniques, detailed below, including various spectroscopy and microscopy approaches.

• • UV-Visible-NIR absorption spectroscopy [Shimadzu 2450 and Perkin Elmer Lambda 950]

  • Fluorescence spectroscopy [Horiba Fluorolog-3 & Agilent Cary Eclipse]

  • Dynamic light scattering (DLS) [Malvern Zetasizer]

  • Time-resolved fluorescence spectroscopy [Horiba DeltaFlex & PicoQuant’s PicoHarp]

a)   Fluorescence lifetime spectroscopy

b)  Fluorescence anisotropy

• Relative and absolute quantum yield analysis [Horiba Fluorolog-3 + Quanta-φ integrating sphere]

• High-resolution confocal laser scanning microscopy (CLSM) [Nikon Confocal microscope + PicoQuant Laser scanning module (LSM) upgrade kit for time resolved confocal microscopy]

a)   Confocal Imaging

b)  Confocal based Super Resolution Radial Fluctuation (SRRF) microscopy

c)   Super resolution SRRF [non-laser illumination + EMCCD for signal capturing]

d)  Fluorescence correlation spectroscopy (FCS)

e)   Fluorescence lifetime imaging microscopy (FLIM)

• Single molecule/particle spectroscopy (intensity-time traces) with home-built setup [4 Laser in TIRF and widefield mode with Andor iXon ultra 897U back illuminated EMCCD]

• Super-resolution microscopy with custom-built setup

a)   Stochastic optical reconstruction microscopy (STORM)

b)  SRRF

• Design and synthesis of various nanomaterials, including quantum dots (QDs) of different sizes, compositions, and structures via aqueous and hot injection synthesis methods, carbon nanodots, gold nanoparticles, silica nanoparticles, etc.

• Expertise in Data Analysis with developing batch analysis tools using programming language (ImageJ and its macro language, MATLAB).